Tips and Tricks for EM

Storing high pressure frozen sample prior to freeze substitutionHigh Pressure Freezing is, in 90% of the cases, followed by Freeze Substitution.However, we never considered the FS procedure to be reliable, reproducible an...

Biopsy preparation procedure for vitrification by High Pressure FreezingIn this video, we illustrate the preparation of a 100µm thin slice of a 4%PFA perfused mouse liver, using first an oscilating blade microtome.The slice is th...

Estimate your temperature shift between your FS setting and your sample - instructions

Excel sheet to caculate simply the correction required

A good ressource website for EM images of a wide variety of sample

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