FS baskets

Storing high pressure frozen sample prior to freeze substitutionHigh Pressure Freezing is, in 90% of the cases, followed by Freeze Substitution.However, we never considered the FS procedure to be reliable, reproducible an...

Opening the HPF sandwich

This video illustrates how we open the HPF sandwich to select the carrier containing the sample. Then we deposit the sandwich in the FS basket for freeze-substitution processing.

Handling the freeze substitution basket

Exchanging Fluids during FS

How to increase freeze-substitution and electron microscopy embedding reliabilityFreeze substitution is a crucial technique in electron microscopy, enabling the preservation of biological specimen ultrastructure. However, existing tools are primarily designed for 3 mm carriers, limiting their compatibility with larger carriers such as CryoCapsules or 6 mm carriers. In this study, we developed a novel freeze substitution tool, simply named the FS-basket, to address the challenges associated with embedding samples vitrified in larger carriers. The tool comprises a mesh with pore sizes smaller than the sample chunks, deposited below the carrier to maintain a tight seal while allowing for easy fluid exchange.

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